Journal: International Journal of Molecular Medicine

Article Title: Kcnh2 deletion is associated with rat embryonic development defects via destruction of KCNH2-integrin β1 complex

doi: 10.3892/ijmm.2023.5338

Figure Lengend Snippet: Generation of Kcnh2 mutant rats by homologous recombination. (A) Schematic diagram showing the WT allele of rat Kcnh2 gene, the Kcnh2 targeting vector and the targeted Kcnh2 gene locus. (B) PCR screening results for drug-resistant clones using the primers shown in (A). The expected product of 2.6 kb is indicated by an arrow. The asterisks indicate the clones picked for the next step. (C) Southern blot analysis for Kcnh2 gene-targeted ES cells in the selected 4 clones from (B). The genomic DNA was digested by Bgl II and the 3′probe indicated in (A) was used for blotting. Among the four rESCs clones, three of them were correctly targeted as indicated by the asterisks. (D) Germline pups were generated by crossing the male chimaera with a WT Sprague-Dawley female rat. The expression of GFP signified that the targeted allele was transmitted to the pups. (E) Diagrams showing the positions of three PCR primers designed to genotype Kcnh2 targeted pups. (F) Genotyping results of germline pups from (E). All GFP-positive pups generated two bands, which indicates that all of them are heterozygous and the Kcnh2 targeted allele was transmitted. All primer sequences are shown in . sc-F/sc-R, genotype primers used for screening target ES clones; 3′probe used for southern blotting; F1/F2/R′, primers used for genotype of rats; M, DNA marker.

Article Snippet: A total of four female Fischer 344 rats, five male Fischer 344 rats, four female Sprague-Dawley rats and five male vasectomized Sprague-Dawley rats were purchased from Envigo for performing Kcnh2 knockout.

Techniques: Mutagenesis, Homologous Recombination, Plasmid Preparation, Clone Assay, Southern Blot, Generated, Expressing, Marker

Journal: Toxicological Sciences

Article Title: The Long-Lasting Rodenticide Brodifacoum Induces Neuropathology in Adult Male Rats

doi: 10.1093/toxsci/kfx134

Figure Lengend Snippet: Kaplan Meier and Dixon plot analysis of acute BDF toxicity. A, Adult male Sprague Dawley rats (about 300 gm) were administered 300 μg/kg BDF (p.o. in 500 μl saline by gavage). Survival was monitored for up to 7 days at which time all animals (n = 16) had died or had reached endpoint criteria.B, Adult male Sprague Dawley rats were administered the indicated doses of BDF (p.o. in 500 μl saline) after which survival was monitored up to 7 days. The x-axis “Trial” indicates each individual test that was done at the indicated BDF concentration. Dixon analysis yields the 4 day LD50 as 221 ± 14 μg/kg.

Article Snippet: In brief, Sprague Dawley rat red blood cells (RBCs) were purchased from Innovative Research (Novi, Michigan) and kept at 4 °C.

Techniques: Concentration Assay

Journal: Toxicological Sciences

Article Title: The Long-Lasting Rodenticide Brodifacoum Induces Neuropathology in Adult Male Rats

doi: 10.1093/toxsci/kfx134

Figure Lengend Snippet: Effects of BDF on hematological properties. Adult male Sprague Dawley rats were administered 250 μg/kg (the oral LD50 dose) of BDF or the equivalent amount of vehicle. After 1 and 4 days, blood samples were taken for analysis of (A) hematocrit, (B) RBCs, (C) hemoglobin, and (D) PLT and MPV. Data are mean ± SEM of 4 rats per group (vehicle or BDF treated). *p < .05 versus corresponding vehicle group on the same day. E, Representative image of the small intramucossal hemorrhages that were observed in the BDF-treated rats upon necropsy. F, Hemolysis was measured after addition of the indicated concentrations of BDF or vehicle to packed RBCs. Comparison is made to addition of PBS alone. Data is mean ± SEM of n = 4 samples per group.

Article Snippet: In brief, Sprague Dawley rat red blood cells (RBCs) were purchased from Innovative Research (Novi, Michigan) and kept at 4 °C.

Techniques: